|
| Status |
Public on Sep 02, 2009 |
| Title |
Rb cKO 3 |
| Sample type |
RNA |
| |
|
| Source name |
Sertoli cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Rb1 flox/-; Amh-Cre +
strain: C57B6J/129SvEv
age: 6 weeks
cell type: Sertoli cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested immediately following fluorescence activated sorting (FACSAriaII cell sorter, BD). Total RNA was extracted using Rneasy Micro Kit (Qiagen). RNA quality was inspected using the QIAxcel System (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The image data were compiled into a project using Beadstudio software gene expression module version 3.2.7. No normalization was used. The data was exported as txt files using the build-in function of export to GeneSpring GX format. The option of sample probe profile was chosen. Data were normalized by shifting to 75 percentile and baseline transformed to the median of all samples.
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Sep 01, 2009 |
| Contact name |
Martin M Matzuk |
| E-mail(s) |
mmatzuk@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Pathology
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE17904 |
Whole genome analysis of Sertoli cell gene expression by retinoblastoma-1 |
|
