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Sample GSM447184 Query DataSets for GSM447184
Status Public on Sep 02, 2009
Title Control 3
Sample type RNA
 
Source name Sertoli cells
Organism Mus musculus
Characteristics genotype: Rb1 flox/-
strain: C57B6J/129SvEv
age: 6 weeks
cell type: Sertoli cell
Extracted molecule total RNA
Extraction protocol Cells were harvested immediately following fluorescence activated sorting (FACSAriaII cell sorter, BD). Total RNA was extracted using Rneasy Micro Kit (Qiagen). RNA quality was inspected using the QIAxcel System (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
Data processing The image data were compiled into a project using Beadstudio software gene expression module version 3.2.7. No normalization was used. The data was exported as txt files using the build-in function of export to GeneSpring GX format. The option of sample probe profile was chosen. Data were normalized by shifting to 75 percentile and baseline transformed to the median of all samples.
 
Submission date Sep 01, 2009
Last update date Sep 01, 2009
Contact name Martin M Matzuk
E-mail(s) mmatzuk@bcm.edu
Organization name Baylor College of Medicine
Department Pathology
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6887
Series (1)
GSE17904 Whole genome analysis of Sertoli cell gene expression by retinoblastoma-1

Data table header descriptions
ID_REF
VALUE signal data were normalized by 75 percentile shift and baseline transformed to the median of all samples

Data table
ID_REF VALUE
ILMN_2659724 -0.34237528
ILMN_2713160 -0.28102565
ILMN_1219601 -0.28661537
ILMN_1252621 -1.1896913
ILMN_2574839 -0.14635944
ILMN_3162407 -0.9738889
ILMN_2514723 0.108091116
ILMN_2692952 -0.29609704
ILMN_1257952 0.7494528
ILMN_1236559 -0.34873152
ILMN_2910076 -0.4319067
ILMN_1240428 -0.12879491
ILMN_2459211 -0.30028272
ILMN_2718462 0.025622845
ILMN_2882078 -0.16248894
ILMN_2882079 -0.04081726
ILMN_2570659 -0.5529275
ILMN_2465544 -0.22811818
ILMN_2724039 -0.08162904
ILMN_2598594 -1.1388054

Total number of rows: 45281

Table truncated, full table size 1082 Kbytes.




Supplementary file Size Download File type/resource
GSM447184_3920.txt.gz 651.3 Kb (ftp)(http) TXT
Processed data included within Sample table

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