|
| Status |
Public on Dec 15, 2012 |
| Title |
THP-1_PBS_4 |
| Sample type |
RNA |
| |
|
| Source name |
THP-1 monocytes, PBS, 24hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: THP-1 monocytic cell line
agent (stimulus): PBS
|
| Treatment protocol |
THP-1 cells were stimulated with LPS (100ng/mL) or TGFβ1 (1ng/mL) or LPS and TGFβ1 for 24 hrs and kept in an CO2 injecting incubator. As a control PBS was added to the cells.
|
| Growth protocol |
THP-1 cells were cultured in RPMI-1640 medium containing 50mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5% FBS. Monocytes, in a density of 2*10^6 cells/4 mL were starved O/N prior to stimulation in medium containing 0.1% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of stimulated monocytes was isolated using Tripure reagent (Roche) according the manufacturer’s protocol. High quality RNA was obtained with NucleoSpin RNA II kits (Machery-Nagel) inclusive DNase treatment
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kits were used for cDNA and cRNA synthesis (Ambion) according the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
According to beadchip array manufacturer's protocol
|
| Scan protocol |
According to beadchip array manufacturer's protocol
|
| Description |
4223164097_B
|
| Data processing |
Gene expression values of the chips were normalized in BeadStudio (version 3.2 Illumina)
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Dec 15, 2012 |
| Contact name |
Karlijn van Keulen |
| E-mail(s) |
j.k.vankeulen@umcutrecht.nl
|
| Phone |
0031307557155
|
| Organization name |
University Medical Center Utrecht
|
| Department |
division heart and lungs
|
| Lab |
Experimental Cardiology
|
| Street address |
Heidelberglaan 100, G02.523
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CX |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE17909 |
Effect of simultaneous activation of TGFbeta and TLR4 pathway in human monocytes |
|
