|
| Status |
Public on Oct 30, 2009 |
| Title |
Carcasses 1 round amplification, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
carcasses isolated from 20 Anopheles gambiae female adults, G3 strain
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: Carcass
treatment protocol: Carcasses (mosquito tissues after hemocyte collection and decapitation) were harvested from 20 females on ice. Heads were collected into 200ul Trizol on ice, flash frozen in liquid nitrogen, and stored at -80ºC
rna extraction method: Trizol extraction, clean-up with RNeasy Mini protocol (Qiagen)
labeling protocol: One cycle Eukaryotic labeling protocol (affymetrix)
starting amount of total rna: 1000 ng
strain: G3
|
| Treatment protocol |
All tissues were isolated from naïve female mosquitoes 1-2 days after eclosion and stored in their respective solutions at -80ºC. For specific treatment protocol, see Samples section
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was cleaned up according to the RNeasy Mini protocol for animal cells (Qiagen), for specific extract protocol see Samples section
|
| Label |
biotin
|
| Label protocol |
Total RNA was biotinylated according to the Eukaryotic Target Labeling protocol (Affymetrix), for specific labeling protocol see Samples section
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression data from carcasses isolated from Anopheles gambiae female adults 1-2 days after eclosion
|
| Data processing |
GCOS 1.4
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Sep 30, 2009 |
| Contact name |
Kristin Michel |
| E-mail(s) |
kmichel@ksu.edu
|
| Organization name |
Kansas State University
|
| Department |
Biology
|
| Street address |
267 Chalmers Hall
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE17919 |
Expression profiling of different adult female tissues isolated from Anopheles gambiae females |
|
