|
| Status |
Public on Oct 30, 2009 |
| Title |
Hemocytes 2 round amplification, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Hemocytes isolated from 1000 Anopheles gambiae female adults, G3 strain
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: Circulating hemocytes
treatment protocol: Hemocytes were harvested from 500 infected females by proboscis clipping and slight thorax-squeezing with dissection forceps. A hemolymph drop from each mosquito was collected directly into RNeasy’s buffer RLT (Qiagen) and stored at -80ºC
rna extraction method: RNeasy Mini protocol for animal cells (Qiagen)
labeling protocol: Two cycle Eukaryotic labeling protocol (affymetrix)
starting amount of total rna: 80 ng
strain: G3
|
| Treatment protocol |
All tissues were isolated from naïve female mosquitoes 1-2 days after eclosion and stored in their respective solutions at -80ºC. For specific treatment protocol, see Samples section
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was cleaned up according to the RNeasy Mini protocol for animal cells (Qiagen), for specific extract protocol see Samples section
|
| Label |
biotin
|
| Label protocol |
Total RNA was biotinylated according to the Eukaryotic Target Labeling protocol (Affymetrix), for specific labeling protocol see Samples section
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression data from circulating hemocytes isolated from Anopheles gambiae female adults 1-2 days after eclosion
|
| Data processing |
GCOS 1.4
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Sep 30, 2009 |
| Contact name |
Kristin Michel |
| E-mail(s) |
kmichel@ksu.edu
|
| Organization name |
Kansas State University
|
| Department |
Biology
|
| Street address |
267 Chalmers Hall
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE17919 |
Expression profiling of different adult female tissues isolated from Anopheles gambiae females |
|
