|
| Status |
Public on Jan 28, 2010 |
| Title |
A/J Amygdala (1855537005_E) |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Amygdala
|
| Organism |
Mus musculus |
| Characteristics |
age: 3-4 months
gender: M
strain: A/J
brain region: Amygdala
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Concentrations were det
|
| Label |
biotin
|
| Label protocol |
RNA samples were prepared with the Illumina TotalPrep kit amplification and labeling protocol. Amplified and biotinylated CRNA was measured with a ribogreen assay
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
1855537005_E
|
| Data processing |
Data were backgroundcorrected in Beadstudio and transformed (vst) and normalised (rsn) in Lumi (R package)
|
| |
|
| Submission date |
Sep 03, 2009 |
| Last update date |
May 16, 2011 |
| Contact name |
Simone de Jong |
| E-mail(s) |
s.dejong-6@umcutrecht.nl
|
| Organization name |
UMC Utrecht
|
| Department |
Medical Genetics
|
| Street address |
Universiteitsweg 100
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CG |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE17955 |
Amygdalae and hippocampi of C57BL/6J and A/J mouse inbred strains |
|
