|
Status |
Public on Sep 04, 2012 |
Title |
young non-growing oocyte-1 |
Sample type |
RNA |
|
|
Source name |
young non-growng oocyte-1
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 6-8 weeks old tissue: oocytes developmental stage: promordial follicle (20-50um diameter)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were isolated using mirVana miRNA Isolation Kit (Applied Biosystems).
|
Label |
cyanine 3
|
Label protocol |
according to manufacturer protocol
|
|
|
Hybridization protocol |
according to manufacturer protocol
|
Scan protocol |
Microarray slides were scanned at 5-um resolution using an Agilent microarray scanner G2565BA (Agilent technology).
|
Description |
Young non-growing oocyte-1 were derived from 6-8-week-old C57BL/6 mice. The oocytes were selected as the diameter of smaller than 60 um.
|
Data processing |
Exactly 100ng of total RNA were used for each samples. The resulting images were quantified using Agilent Feature Extraction Software V 9.5. The signal =< 10 and negative were normalized to 0.
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|
|
Submission date |
Sep 04, 2009 |
Last update date |
Sep 04, 2012 |
Contact name |
Maki Kusumi |
E-mail(s) |
kusumim-tky@umin.net
|
Phone |
+81-3-3416-0181
|
Fax |
+81-3-3417-2864
|
Organization name |
National Research Institute for Child Health and Development
|
Department |
Maternal-Fetal Biology
|
Street address |
2-10-1 Okura
|
City |
Setagaya |
State/province |
Tokyo |
ZIP/Postal code |
157-8535 |
Country |
Japan |
|
|
Platform ID |
GPL8824 |
Series (1) |
GSE17966 |
oocyte miRNA profiling by array |
|