|
| Status |
Public on Sep 04, 2012 |
| Title |
young non-growng oocyte-2 |
| Sample type |
RNA |
| |
|
| Source name |
young non-growing oocyte-2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6-8 weeks old
tissue: oocytes
developmental stage: promordial follicle (20-50um diameter)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using mirVana miRNA Isolation Kit (Applied Biosystems).
|
| Label |
cyanine 3
|
| Label protocol |
according to manufacturer protocol
|
| |
|
| Hybridization protocol |
according to manufacturer protocol
|
| Scan protocol |
Microarray slides were scanned at 5-um resolution using an Agilent microarray scanner G2565BA (Agilent technology).
|
| Description |
Young non-growing oocyte-2 were derived from 6-8-week-old C57BL/6 mice. The oocytes were selected as the diameter of smaller than 60 um.
|
| Data processing |
Exactly 100ng of total RNA were used for each samples. The resulting images were quantified using Agilent Feature Extraction Software V 9.5. The signal =< 10 and negative were normalized to 0.
|
| |
|
| Submission date |
Sep 04, 2009 |
| Last update date |
Sep 04, 2012 |
| Contact name |
Maki Kusumi |
| E-mail(s) |
kusumim-tky@umin.net
|
| Phone |
+81-3-3416-0181
|
| Fax |
+81-3-3417-2864
|
| Organization name |
National Research Institute for Child Health and Development
|
| Department |
Maternal-Fetal Biology
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE17966 |
oocyte miRNA profiling by array |
|
