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Sample GSM44979 Query DataSets for GSM44979
Status Public on Mar 23, 2005
Title CD4+ CD25- Foxp3- T cells
Sample type RNA
Source name mouse CD4+ T cells
Organism Mus musculus
Extracted molecule total RNA
Description Mice used were Foxp3-GFP knock-in B6/129 F2's of 5-8 weeks of age. Single cell suspensions of cells from spleens and lymph nodes of 25 male mice were generated. Cell suspensions were enriched for CD4+ T cells by magnetic antibody mediated depletion using a cocktail of biotinylated antibodies specific for CD8, B220, CD11b, NK1.1, and DX5 followed by anti-biotin MACS beads. The remaining cells were labeled with CD4-PerCP, CD25-APC antibodies, and PE-conjugated streptavidin. CD4+PE- cells were sorted into four populations based on expression of GFP and CD25. Total RNA was isolated and biotinylated antisense cRNA was prepared using two cycles of in vitro amplification according to the Affymetrix Small Sample Labeling Protocol II. Biotinylated cRNA was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays.
Keywords = regulatory T cells
Keywords = Foxp3
Submission date Mar 08, 2005
Last update date Aug 28, 2018
Contact name Jeffrey Rasmussen
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platform ID GPL1261
Series (1)
GSE2389 Regulatory T cell lineage specification by Foxp3
Reanalyzed by GSE119085

Data table header descriptions
VALUE 2^(gcrma signal) v1.1.3
RAW_VALUE unnormalized MAS 5.0 signal

Data table
1415670_at 2490.473243 831
1415671_at 816.8824187 453.9
1415672_at 1761.560551 688.7
1415673_at 831.0784047 393.3
1415674_a_at 463.1807856 308.1
1415675_at 452.702533 313.3
1415676_a_at 2724.016425 1177.8
1415677_at 271.5782298 83.7
1415678_at 1157.961639 740.4
1415679_at 2899.533156 1645.6
1415680_at 1268.052258 534.9
1415681_at 485.7548992 235.3
1415682_at 253.6454866 129
1415683_at 2116.262118 1289.7
1415684_at 183.9502943 102.6
1415685_at 349.5881475 145.6
1415686_at 363.7482716 222.6
1415687_a_at 5017.255253 1602.3
1415688_at 1491.144307 1000.7
1415689_s_at 584.7516068 336

Total number of rows: 45101

Table truncated, full table size 1229 Kbytes.

Supplementary file Size Download File type/resource
GSM44979.CEL.gz 5.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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