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Sample GSM44980 Query DataSets for GSM44980
Status Public on Mar 23, 2005
Title CD4+ CD25hi Foxp3+ T cells
Sample type RNA
Source name mouse CD4+ T cells
Organism Mus musculus
Extracted molecule total RNA
Description Mice used were Foxp3-GFP knock-in B6/129 F2's of 5-8 weeks of age. Single cell suspensions of cells from spleens and lymph nodes of 25 male mice were generated. Cell suspensions were enriched for CD4+ T cells by magnetic antibody mediated depletion using a cocktail of biotinylated antibodies specific for CD8, B220, CD11b, NK1.1, and DX5 followed by anti-biotin MACS beads. The remaining cells were labeled with CD4-PerCP, CD25-APC antibodies, and PE-conjugated streptavidin. CD4+PE- cells were sorted into four populations based on expression of GFP and CD25. Total RNA was isolated and biotinylated antisense cRNA was prepared using two cycles of in vitro amplification according to the Affymetrix Small Sample Labeling Protocol II. Biotinylated cRNA was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays.
Keywords = regulatory T cells
Keywords = Foxp3
Submission date Mar 08, 2005
Last update date Aug 28, 2018
Contact name Jeffrey Rasmussen
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platform ID GPL1261
Series (2)
GSE2389 Regulatory T cell lineage specification by Foxp3
GSE4179 A function for interleukin 2 in Foxp3-expressing regulatory T cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE 2^(gcrma signal) v1.1.3
RAW_VALUE unnormalized MAS 5.0 signal

Data table
1415670_at 2350.125293 1370.2
1415671_at 642.1214186 764
1415672_at 2147.355083 1427.5
1415673_at 1035.23339 632.4
1415674_a_at 475.6639243 632.7
1415675_at 498.5658766 544.2
1415676_a_at 3604.03312 2231.7
1415677_at 309.4322073 196.6
1415678_at 1108.965057 1199.9
1415679_at 2882.954571 3040.5
1415680_at 1259.07727 974
1415681_at 538.8792723 438.2
1415682_at 316.3170162 325.9
1415683_at 2152.071424 2206.8
1415684_at 192.8822766 155
1415685_at 605.1130206 420.3
1415686_at 468.9657397 463
1415687_a_at 5042.6642 2756.4
1415688_at 1410.960676 1738.6
1415689_s_at 759.7727594 846.6

Total number of rows: 45101

Table truncated, full table size 1237 Kbytes.

Supplementary file Size Download File type/resource
GSM44980.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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