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Sample GSM44981 Query DataSets for GSM44981
Status Public on Mar 23, 2005
Title CD4+ CD25lo Foxp3+ T cells
Sample type RNA
Source name mouse CD4+ T cells
Organism Mus musculus
Extracted molecule total RNA
Description Mice used were Foxp3-GFP knock-in B6/129 F2's of 5-8 weeks of age. Single cell suspensions of cells from spleens and lymph nodes of 25 male mice were generated. Cell suspensions were enriched for CD4+ T cells by magnetic antibody mediated depletion using a cocktail of biotinylated antibodies specific for CD8, B220, CD11b, NK1.1, and DX5 followed by anti-biotin MACS beads. The remaining cells were labeled with CD4-PerCP, CD25-APC antibodies, and PE-conjugated streptavidin. CD4+PE- cells were sorted into four populations based on expression of GFP and CD25. Total RNA was isolated and biotinylated antisense cRNA was prepared using two cycles of in vitro amplification according to the Affymetrix Small Sample Labeling Protocol II. Biotinylated cRNA was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays.
Keywords = regulatory T cells
Keywords = Foxp3
Submission date Mar 08, 2005
Last update date Aug 28, 2018
Contact name Jeffrey Rasmussen
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platform ID GPL1261
Series (2)
GSE2389 Regulatory T cell lineage specification by Foxp3
GSE4179 A function for interleukin 2 in Foxp3-expressing regulatory T cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE 2^(gcrma signal) v1.1.3
RAW_VALUE unnormalized MAS 5.0 signal

Data table
1415670_at 1962.771382 1199.1
1415671_at 836.2652529 863.1
1415672_at 2796.778131 1814.4
1415673_at 993.2254417 584.9
1415674_a_at 446.7502936 537.7
1415675_at 451.1416651 491.1
1415676_a_at 2991.229356 1923.4
1415677_at 487.2980056 309.3
1415678_at 998.3827722 1019
1415679_at 2923.907525 2885
1415680_at 1168.286842 854.5
1415681_at 491.0719524 454.9
1415682_at 420.9713174 352.6
1415683_at 2199.894733 2077.2
1415684_at 228.2593392 185.3
1415685_at 624.6354285 460.5
1415686_at 494.7676021 496.6
1415687_a_at 5119.65785 2728.7
1415688_at 1369.308911 1590.6
1415689_s_at 921.6817973 908.3

Total number of rows: 45101

Table truncated, full table size 1239 Kbytes.

Supplementary file Size Download File type/resource
GSM44981.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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