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Sample GSM449812 Query DataSets for GSM449812
Status Public on Sep 05, 2009
Title Liver iron diet rep1
Sample type RNA
 
Source name Mouse liver, iron supplemented diet
Organism Mus musculus
Characteristics gender: male
strain: C57BL/6
tissue: liver
age: 16-18 weeks
Extracted molecule total RNA
Extraction protocol RNA was extracted with RNeasy RNA isolation kit including RNase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
Label biotin
Label protocol Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion) was used following the manufacturer's protocol. Input amount was 300ng total RNA and duration of labeling reaction incubation was 14 hrs.
 
Hybridization protocol Standard Illumina hybridization protocol (18h at 55 degrees) was used).
Scan protocol Standard scan factor in Illumina BeadArray reader was used.
Description no additional information
Data processing Array data were normalized with Chipster version 1.1.1 using the quantile normalization method.
 
Submission date Sep 04, 2009
Last update date Sep 04, 2009
Contact name Alejandra Rodriguez-Martinez
E-mail(s) alejandra.rodriguez.martinez@uta.fi
Organization name University of Tampere
Department Institute of Biomedical Technology
Street address Biokatu 6
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6105
Series (1)
GSE17969 Effects of Hfe-/- and dietary iron overload on gene expression in the liver and duodenum of mice

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
ILMN_2735294 7.95 0
ILMN_2417611 7.91 0
ILMN_2545897 6.53 0.02495321
ILMN_1238136 6.29 0.2164691
ILMN_1248788 6.28 0.2376793
ILMN_2707227 13.06 0
ILMN_2721178 7.74 0
ILMN_1227723 7.63 0
ILMN_2458840 6.15 0.5427324
ILMN_2729028 6.14 0.5608234
ILMN_2602282 6.07 0.724267
ILMN_2624326 8.8 0
ILMN_1230777 10.9 0
ILMN_2730714 11.16 0
ILMN_1246069 10.03 0
ILMN_2524361 6.36 0.1166563
ILMN_1243193 9.07 0
ILMN_1232042 6.14 0.5533375
ILMN_2543689 7.13 0
ILMN_1233188 6.25 0.2888334

Total number of rows: 46632

Table truncated, full table size 1219 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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