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Sample GSM44982 Query DataSets for GSM44982
Status Public on Mar 23, 2005
Title CD4+ CD25+ Foxp3- T cells
Sample type RNA
Source name mouse CD4+ T cells
Organism Mus musculus
Extracted molecule total RNA
Description Mice used were Foxp3-GFP knock-in B6/129 F2's of 5-8 weeks of age. Single cell suspensions of cells from spleens and lymph nodes of 25 male mice were generated. Cell suspensions were enriched for CD4+ T cells by magnetic antibody mediated depletion using a cocktail of biotinylated antibodies specific for CD8, B220, CD11b, NK1.1, and DX5 followed by anti-biotin MACS beads. The remaining cells were labeled with CD4-PerCP, CD25-APC antibodies, and PE-conjugated streptavidin. CD4+PE- cells were sorted into four populations based on expression of GFP and CD25. Total RNA was isolated and biotinylated antisense cRNA was prepared using two cycles of in vitro amplification according to the Affymetrix Small Sample Labeling Protocol II. Biotinylated cRNA was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays.
Keywords = regulatory T cells
Keywords = Foxp3
Submission date Mar 08, 2005
Last update date Aug 28, 2018
Contact name Jeffrey Rasmussen
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platform ID GPL1261
Series (2)
GSE2389 Regulatory T cell lineage specification by Foxp3
GSE4179 A function for interleukin 2 in Foxp3-expressing regulatory T cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE 2^(gcrma signal) v1.1.3
RAW_VALUE unnormalized MAS 5.0 signal

Data table
1415670_at 1921.409333 1230.3
1415671_at 543.2982735 602.8
1415672_at 2712.276185 2119.8
1415673_at 1129.98178 706.1
1415674_a_at 462.3568072 604.7
1415675_at 328.7793192 427.7
1415676_a_at 3884.630951 2587.4
1415677_at 206.6011712 116.8
1415678_at 857.6405872 912.9
1415679_at 1610.624747 2107
1415680_at 1261.818228 1077.7
1415681_at 503.6199314 504.8
1415682_at 393.9584523 376.2
1415683_at 2456.612595 2257.4
1415684_at 177.9056257 184.8
1415685_at 665.6539515 562.5
1415686_at 431.9328782 483.1
1415687_a_at 4104.458271 2380.8
1415688_at 1285.794754 1589.6
1415689_s_at 695.6498543 767.9

Total number of rows: 45101

Table truncated, full table size 1239 Kbytes.

Supplementary file Size Download File type/resource
GSM44982.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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