|
| Status |
Public on Sep 05, 2009 |
| Title |
Duodenum wt rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse duodenum
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6
tissue: duodenum
age: 13 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNeasy RNA isolation kit including RNase-free Dnase treatment (Qiagen) according to the manufacturer's protocol. Concentration and purity were measured with Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion) was used following the manufacturer's protocol. Input amount was 300ng total RNA and duration of labeling reaction incubation was 14 hrs.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol (18h at 55 degrees) was used).
|
| Scan protocol |
Standard scan factor in Illumina BeadArray reader was used.
|
| Description |
no additional information
|
| Data processing |
Array data were normalized with Chipster version 1.1.1 using the quantile normalization method.
|
| |
|
| Submission date |
Sep 04, 2009 |
| Last update date |
Sep 04, 2009 |
| Contact name |
Alejandra Rodriguez-Martinez |
| E-mail(s) |
alejandra.rodriguez.martinez@uta.fi
|
| Organization name |
University of Tampere
|
| Department |
Institute of Biomedical Technology
|
| Street address |
Biokatu 6
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE17969 |
Effects of Hfe-/- and dietary iron overload on gene expression in the liver and duodenum of mice |
|
