|
| Status |
Public on Aug 31, 2021 |
| Title |
genomic DNA from unstable plaque 1 |
| Sample type |
genomic |
| |
|
| Source name |
unstable atherosclerotic plaque 1
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
sample type: unstable atherosclerotic plaque
disease state: unstable lesion
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from frozen tissue specimens using the QIAamp DNA Mini Kit (Qiagen) following the manufacturer’s instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 and Methylation850 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
unstable atherosclerotic plaque
unstable1
|
| Data processing |
Array data were evaluated and preprocessed using the minfi R package (version 1.32.0). The 450K and EPIC data were merged with the combineArrays function, the batch effect was corrected with sva R package (version3.34.0)
|
| |
|
| Submission date |
May 02, 2020 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Hang Yang |
| E-mail(s) |
hangyang@genetics.ac.cn
|
| Organization name |
CAS
|
| Street address |
No.1 West Beichen Road,Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL16304 |
| Series (1) |
| GSE149759 |
Genome-wide DNA methylation profiling in Atherosclerotic plaques |
|
