|
| Status |
Public on Nov 18, 2021 |
| Title |
day9, differentiated adipocyte (Tmem120a+Tmem120b knock-down), rep2 |
| Sample type |
RNA |
| |
|
| Source name |
3T3L1 preadipocyte cell line
|
| Organism |
Mus musculus |
| Characteristics |
time: day 9
treatment: differentiation
genotype/variation: Tmem120a/Tmem120b knock-down
|
| Treatment protocol |
Adipogenesis was induced two days post-confluency by replacing growth medium with induction medium containing 0.5 μM insulin, 1 µM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), and 1 μM rosiglitazone. Induction medium was replaced after two days with maintenance medium containing growth medium supplemented with insulin and rosiglitazone only, which was replaced every 48h, up to day 9 post-induction.
Control and Tmem120a/b knock-downs were generated by transduction of non-target (control) or Tmem120a/b-specific shRNA-encoding lentiviruses as described in Batrakou et al. (2015) PLoS ONE 10(05): e0127712.
|
| Growth protocol |
3T3-L1 cells were maintained in high glucose DMEM (SIGMA) supplemented with 10% fetal bovine serum (FBS), MEM non-essential amino acids, 1 mM Sodium Pyruvate, 100 µg/µl penicillin and 100 µg/µl streptomycin sulfate. Cells were plated at ~10% confluency and always split by 60% confluency to maintain differentiation capacity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction was performed using Trizol reagent (Invitrogen) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Ambion Illumina TotalPrep kit
|
| |
|
| Hybridization protocol |
Standard Protocols (Clinical Research Facility Edinburgh)
|
| Scan protocol |
Standard Protocols (Clinical Research Facility Edinburgh), Scanner: HiScan H166
|
| Description |
replicate 2
|
| Data processing |
Data were log2 transformed and quantile normalised using the BioConductor package Limma.
|
| |
|
| Submission date |
May 07, 2020 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Jose Ignacio de las Heras |
| E-mail(s) |
j.delasheras@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Trust Centre
|
| Lab |
Swann 5.12
|
| Street address |
Max Born Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE150022 |
Transcriptome analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b |
| GSE150507 |
Analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b |
|
