|
| Status |
Public on Dec 18, 2009 |
| Title |
Colon_Embryo_Day21_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
embryo, day 21, colon
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
age: embryo, day 21
tissue: colon
|
| Treatment protocol |
tissue was snap frozen in liquid nitrogen and stored at -80°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified with the RNAdvance tissue kit (Agencourt) extraction. RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on an Agilent 2100 Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA was synthesized, labelled and purified according to the Illumina TotalPrep RNA amplification protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 5
|
| Data processing |
Quality control of the data was performed on all samples with a Pearson correlation matrix and a Principal Component Analysis. The data were normalised using quantile normalisation with IlluminaGUI
|
| |
|
| Submission date |
Sep 16, 2009 |
| Last update date |
Dec 18, 2009 |
| Contact name |
Peter I Duncan |
| E-mail(s) |
peter.duncan@rdls.nestle.com
|
| Organization name |
Nestlé Research Center
|
| Department |
Nutrition & Health
|
| Lab |
Digestive Health
|
| Street address |
Vers-chez-les-Blanc
|
| City |
Lausanne |
| ZIP/Postal code |
1026 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE18133 |
Genome-wide analysis of gene expression in colon and brain during the suckling period. |
|
