NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM453492 Query DataSets for GSM453492
Status Public on Dec 18, 2009
Title Colon_PostNatalDay15_rep6
Sample type RNA
 
Source name colon, day 15
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
age: postnatal day 15
tissue: colon
Treatment protocol tissue was snap frozen in liquid nitrogen and stored at -80°C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified with the RNAdvance tissue kit (Agencourt) extraction. RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on an Agilent 2100 Bioanalyser.
Label Cy3
Label protocol Biotinylated cRNA was synthesized, labelled and purified according to the Illumina TotalPrep RNA amplification protocol.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 6
Data processing Quality control of the data was performed on all samples with a Pearson correlation matrix and a Principal Component Analysis. The data were normalised using quantile normalisation with IlluminaGUI
 
Submission date Sep 16, 2009
Last update date Dec 18, 2009
Contact name Peter I Duncan
E-mail(s) peter.duncan@rdls.nestle.com
Organization name Nestlé Research Center
Department Nutrition & Health
Lab Digestive Health
Street address Vers-chez-les-Blanc
City Lausanne
ZIP/Postal code 1026
Country Switzerland
 
Platform ID GPL6101
Series (1)
GSE18133 Genome-wide analysis of gene expression in colon and brain during the suckling period.

Data table header descriptions
ID_REF
VALUE quantile normalized
C_76.Detection Pval

Data table
ID_REF VALUE C_76.Detection Pval
ILMN_1348778 253.4134 0
ILMN_1348782 412.3127 0
ILMN_1348783 1061.724 0
ILMN_1348784 62.79636 0.1078788
ILMN_1348786 55.81272 0.4351515
ILMN_1348787 47.47884 0.8981818
ILMN_1348789 62.46921 0.1175758
ILMN_1348790 99.6802 0.002424242
ILMN_1348791 115.206 0.002424242
ILMN_1348792 47.13661 0.9103031
ILMN_1348793 59.39091 0.2339394
ILMN_1348794 100.4112 0.002424242
ILMN_1348795 55.14381 0.4678788
ILMN_1348797 51.40051 0.7078788
ILMN_1348798 51.90265 0.6606061
ILMN_1348799 99.29411 0.002424242
ILMN_1348800 550.6384 0
ILMN_1348802 51.75874 0.6787879
ILMN_1348804 79.04185 0.008484849
ILMN_1348806 58.04467 0.299394

Total number of rows: 22523

Table truncated, full table size 660 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap