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Sample GSM453523 Query DataSets for GSM453523
Status Public on Dec 18, 2009
Title Brain_PostNatalDay5_rep1
Sample type RNA
 
Source name brain, right hemisphere, day 5
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
age: postnatal day 5
tissue: brain, right hemisphere
Treatment protocol tissue was snap frozen in liquid nitrogen and stored at -80°C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified with the RNAdvance tissue kit (Agencourt) extraction. RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on an Agilent 2100 Bioanalyser.
Label Cy3
Label protocol Biotinylated cRNA was synthesized, labelled and purified according to the Illumina TotalPrep RNA amplification protocol.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 1
Data processing Quality control of the data was performed on all samples with a Pearson correlation matrix and a Principal Component Analysis. The data were normalised using quantile normalisation with IlluminaGUI
 
Submission date Sep 16, 2009
Last update date Dec 18, 2009
Contact name Peter I Duncan
E-mail(s) peter.duncan@rdls.nestle.com
Organization name Nestlé Research Center
Department Nutrition & Health
Lab Digestive Health
Street address Vers-chez-les-Blanc
City Lausanne
ZIP/Postal code 1026
Country Switzerland
 
Platform ID GPL6101
Series (1)
GSE18133 Genome-wide analysis of gene expression in colon and brain during the suckling period.

Data table header descriptions
ID_REF
VALUE quantile normalized
B_41.Detection Pval

Data table
ID_REF VALUE B_41.Detection Pval
ILMN_1348778 658.2073 0
ILMN_1348782 220.8089 0
ILMN_1348783 75.28535 0.02787879
ILMN_1348784 100.1251 0.004848485
ILMN_1348786 61.05219 0.249697
ILMN_1348787 53.71095 0.6242424
ILMN_1348789 62.72528 0.1975758
ILMN_1348790 74.05534 0.03030303
ILMN_1348791 71.61337 0.04727273
ILMN_1348792 51.58663 0.7527273
ILMN_1348793 57.95829 0.3915152
ILMN_1348794 53.71629 0.6242424
ILMN_1348795 55.57528 0.5224242
ILMN_1348797 55.28177 0.5369697
ILMN_1348798 49.94331 0.8460606
ILMN_1348799 156.8523 0.002424242
ILMN_1348800 72.61507 0.03757576
ILMN_1348802 64.99614 0.1357576
ILMN_1348804 101.7955 0.004848485
ILMN_1348806 77.19976 0.0230303

Total number of rows: 22523

Table truncated, full table size 671 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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