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Status |
Public on Oct 21, 2009 |
Title |
Cells transduced with vector expressing GFP (MCF10A GFP 1 Unselected) |
Sample type |
RNA |
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|
Source name |
MCF10A GFP 1 Unselected
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF10A
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction was performed using Trizol plus RNA purification kit (Invitrogen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
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Label |
biotin-SA Cy3
|
Label protocol |
cRNA synthesis was performed using the Illumina TotalPrep RNA amplification kit introducing the following variations: 20 ug of total RNA using pFB specific primer starting from
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Hybridization protocol |
1500 ng of cRNA was hyb on mouse-6 for 16-20 hours. Array washing was performed using wash buffer; an high strincengy temp wash was performed at 55C for 30 minutes. Arrays were stained with SA-Cy3
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Scan protocol |
The hybridized BeadChips were scanned by Illumina BeadScan scanner and analyzed by Illumina's BeadStudio version 1.5.1.3
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Description |
Transduced with vector expressing GFP
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Data processing |
Data are summarized with beadstudio 1.5.1.3 and intentionally not normalized to preserve the asymmetry due to selection and enrichment of a limited number of transcripts.
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Submission date |
Sep 23, 2009 |
Last update date |
Oct 21, 2009 |
Contact name |
Enzo Medico |
E-mail(s) |
enzo.medico@ircc.it
|
Phone |
+39-011-9933234
|
Organization name |
Candiolo Cancer Institute, University of Torino
|
Department |
Oncology
|
Lab |
Laboratory of Oncogenomics
|
Street address |
Strada Prov. 142, km 3,95
|
City |
Candiolo |
State/province |
TO |
ZIP/Postal code |
10060 |
Country |
Italy |
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Platform ID |
GPL6333 |
Series (1) |
GSE18237 |
Genome functional screening identifies GAB2 as a key promoter of anchorage independence in normal and neoplastic cells |
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