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Sample GSM455814 Query DataSets for GSM455814
Status Public on Oct 21, 2009
Title Cells transduced with vector expressing GFP (MCF10A GFP 1 Unselected)
Sample type RNA
 
Source name MCF10A GFP 1 Unselected
Organism Homo sapiens
Characteristics cell line: MCF10A
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using Trizol plus RNA purification kit (Invitrogen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
Label biotin-SA Cy3
Label protocol cRNA synthesis was performed using the Illumina TotalPrep RNA amplification kit introducing the following variations: 20 ug of total RNA using pFB specific primer starting from
 
Hybridization protocol 1500 ng of cRNA was hyb on mouse-6 for 16-20 hours. Array washing was performed using wash buffer; an high strincengy temp wash was performed at 55C for 30 minutes. Arrays were stained with SA-Cy3
Scan protocol The hybridized BeadChips were scanned by Illumina BeadScan scanner and analyzed by Illumina's BeadStudio version 1.5.1.3
Description Transduced with vector expressing GFP
Data processing Data are summarized with beadstudio 1.5.1.3 and intentionally not normalized to preserve the asymmetry due to selection and enrichment of a limited number of transcripts.
 
Submission date Sep 23, 2009
Last update date Oct 21, 2009
Contact name Enzo Medico
E-mail(s) enzo.medico@ircc.it
Phone +39-011-9933234
Organization name Candiolo Cancer Institute, University of Torino
Department Oncology
Lab Laboratory of Oncogenomics
Street address Strada Prov. 142, km 3,95
City Candiolo
State/province TO
ZIP/Postal code 10060
Country Italy
 
Platform ID GPL6333
Series (1)
GSE18237 Genome functional screening identifies GAB2 as a key promoter of anchorage independence in normal and neoplastic cells

Data table header descriptions
ID_REF
VALUE signal intensity not normalized to preserve the asymmetry due to selection and enrichment of a limited number of transcripts
BEAD_STDEV
Avg_NBEADS
Detection

Data table
ID_REF VALUE BEAD_STDEV Avg_NBEADS Detection
10181072_239_rc-S 159.8 10.7 44 0.39480198
10181072_290-S 197.8 11.1 55 0.86509901
10181072_290_rc-S 206.3 13.6 38 0.91089109
10181072_311-S 220.4 15.8 41 0.94740099
10181072_311_rc-S 144.2 9 46 0.17883663
10181072_418-S 152.8 9.2 52 0.28527228
10181072_418_rc-S 199.4 11.6 40 0.87561881
10181072_486-S 194.4 11.2 43 0.84405941
10181072_486_rc-S 176.1 10.4 37 0.66027228
17974913_3385-S 178 8.9 50 0.68688119
17974913_3385_rc-S 163 9.5 47 0.44306931
17974913_3999_rc-S 107.2 5.1 42 0.00185644
17974913_4071-S 143 12 21 0.16336634
17974913_4071_rc-S 153.9 11.1 37 0.3019802
17974913_4426-S 183 13.1 40 0.73824257
17974913_4426_rc-S 165.6 9.9 41 0.49133663
17974913_4962-S 104.9 6 34 0.00061881
17974913_4962_rc-S 135.1 6.9 36 0.07611386
18S_rRNA_X00686_301-S 39905.7 1243.2 53 1
18S_rRNA_X00686_523-S 17646.7 974 31 1

Total number of rows: 46120

Table truncated, full table size 2013 Kbytes.




Supplementary file Size Download File type/resource
GSM455814_GFP1Uns.xls.gz 1.3 Mb (ftp)(http) XLS
Processed data not applicable for this record

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