|
| Status |
Public on Oct 21, 2009 |
| Title |
Cells transduced with vector expressing Mouse testis library (MCF10A LIB 1 Unselected) |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A LIB 1 Unselected
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using Trizol plus RNA purification kit (Invitrogen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
|
| Label |
biotin-SA Cy3
|
| Label protocol |
cRNA synthesis was performed using the Illumina TotalPrep RNA amplification kit introducing the following variations: 20 ug of total RNA using pFB specific primer starting from
|
| |
|
| Hybridization protocol |
1500 ng of cRNA was hyb on mouse-6 for 16-20 hours. Array washing was performed using wash buffer; an high strincengy temp wash was performed at 55C for 30 minutes. Arrays were stained with SA-Cy3
|
| Scan protocol |
The hybridized BeadChips were scanned by Illumina BeadScan scanner and analyzed by Illumina's BeadStudio version 1.5.1.3
|
| Description |
Transduced with vector expressing Mouse testis library
|
| Data processing |
Data are summarized with beadstudio 1.5.1.3 and intentionally not normalized to preserve the asymmetry due to selection and enrichment of a limited number of transcripts.
|
| |
|
| Submission date |
Sep 23, 2009 |
| Last update date |
Oct 21, 2009 |
| Contact name |
Enzo Medico |
| E-mail(s) |
enzo.medico@ircc.it
|
| Phone |
+39-011-9933234
|
| Organization name |
Candiolo Cancer Institute, University of Torino
|
| Department |
Oncology
|
| Lab |
Laboratory of Oncogenomics
|
| Street address |
Strada Prov. 142, km 3,95
|
| City |
Candiolo |
| State/province |
TO |
| ZIP/Postal code |
10060 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6333 |
| Series (1) |
| GSE18237 |
Genome functional screening identifies GAB2 as a key promoter of anchorage independence in normal and neoplastic cells |
|
