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Sample GSM456587 Query DataSets for GSM456587
Status Public on Nov 04, 2009
Title MEF rep3
Sample type RNA
 
Source name Mouse embryonic fibroblasts (MEFs)
Organism Mus musculus
Characteristics cell type: Embryonic Fibroblast Cells
Treatment protocol Cells were treated with small molecules as described in Ichida et al. (Cell Stem Cell, 2009).
Growth protocol Cells were grown in a monolayer on gelatin-coated plates, either with or without an irradiated mouse embryonic fibroblast (MEF) feeder layer. They were given standard mES media containing either fetal bovine serum (FBS) or knock-out serum replacement (KSR).
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent in accordance with the prescribed protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amp kit.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Non-normalized data file: RAW_2.txt
Data processing The data were normalized using cubic-spline normalization with BeadStudio.
 
Submission date Sep 25, 2009
Last update date Sep 25, 2009
Contact name Esther Yesde Son
E-mail(s) son@fas.harvard.edu
Phone 617-496-5611
Fax 617-384-5611
Organization name Harvard University
Department Stem Cells and Regenerative Biology
Lab Eggan
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE18286 Global gene expression analysis of (1) chemically reprogrammed iPS cells, and (2) chemical-treated cells

Data table header descriptions
ID_REF
VALUE Cubic-spline normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1073.468 0
ILMN_2721178 226.5119 0
ILMN_3033922 614.6472 0
ILMN_3092673 2447.899 0
ILMN_2816356 33.56798 0.002506266
ILMN_2808939 442.9965 0
ILMN_2634564 318.7402 0
ILMN_2737647 -1.210152 0.5150376
ILMN_2734484 389.5314 0
ILMN_2952292 27.01688 0.007518797
ILMN_2699078 -2.54975 0.6491228
ILMN_1213681 63.95299 0
ILMN_2735413 1.279708 0.3558897
ILMN_2735415 3.291609 0.2593985
ILMN_2891688 391.7297 0
ILMN_2637698 553.7538 0
ILMN_2674228 89.05936 0
ILMN_2601546 19.45306 0.01754386
ILMN_1230831 1.555922 0.3433584
ILMN_2848071 19.35805 0.01754386

Total number of rows: 25697

Table truncated, full table size 747 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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