|
| Status |
Public on Jun 15, 2021 |
| Title |
Sample a43446 (Lung, Male), Normal |
| Sample type |
RNA |
| |
|
| Source name |
Lung
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Lung cancer
gender: Male
tissue: Normal
patient id: a43446
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
|
| |
|
| Hybridization protocol |
Hybridization was performed for 16 h on GeneTitanTM Instrument.
|
| Scan protocol |
GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
|
| Description |
Gene expression from surgically resected lung tissue in lung cancer patients.
|
| Data processing |
Gene expression data from the Affymetrix HuGene 1.1 ST array were available for a total of 123 samples from 69 patients with either adenocarcinoma or squamous cell carcinoma. Data were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
|
| |
|
| Submission date |
May 23, 2020 |
| Last update date |
Nov 16, 2021 |
| Contact name |
William Cookson |
| E-mail(s) |
w.cookson@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Street address |
Dovehouse Street
|
| City |
London |
| ZIP/Postal code |
SW3 6LY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11532 |
| Series (2) |
| GSE151102 |
Disruption of Y chromosome expression in male lung cancer: replication |
| GSE151103 |
Disruption of Y chromosome expression in male lung cancer |
|
