|
| Status |
Public on May 19, 2021 |
| Title |
liver tissue_t-DHEA_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
liver tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: liver
treatment: FMT recipient from DHEA treated donor rat
strain: Sprague Dawley
|
| Treatment protocol |
fecal microbiota transplantation
|
| Growth protocol |
specific pathogen-free barrier facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen, Germany) was used to extract total RNA from snap-frozen rat liver tissue.
TruSeq® RNA Sample Preparation Kit (Illumina, USA) was employed to construct paired-end libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
X62
|
| Data processing |
Raw reads were filtered by Seqtk
Hisat2 (version:2.0.4) was employed to map the cleaned reads to Rattus norvegicus Rnor_6.0.
The fragments of genes were counted using Stringtie (version:1.3.0) followed by FPKM normalization.
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: Matrix table with FPKM values and raw gene counts for every gene and every sample
|
| |
|
| Submission date |
May 26, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Qixin HAN |
| E-mail(s) |
Christine-Han@sjtu.edu.cn
|
| Organization name |
Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University
|
| Street address |
NO.845 Lingshan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200135 |
| Country |
China |
| |
|
| Platform ID |
GPL24688 |
| Series (1) |
| GSE151220 |
RNA-seq data of liver samples in FMT recipient rats |
|
| Relations |
| BioSample |
SAMN15020733 |
| SRA |
SRX8401681 |
