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Sample GSM45787 Query DataSets for GSM45787
Status Public on May 14, 2005
Title WT_M_2
Sample type RNA
Source name wild type E12.5 fetal liver derived megakaryocytes
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from wild type embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 855.174 P 8.47842
AFFX-BioB-M_at 1217.52 P 12.8809
AFFX-BioB-3_at 1058.91 P 9.8848
AFFX-BioC-5_at 1633.4 P 25.121
AFFX-BioC-3_at 1783.09 P 24.3004
AFFX-BioDn-5_at 2417.31 P 17.2076
AFFX-BioDn-3_at 6094.11 P 48.4247
AFFX-CreX-5_at 12392.5 P 100.421
AFFX-CreX-3_at 15239.1 P 154.418
AFFX-DapX-5_at 33.8809 A 2.52238
AFFX-DapX-M_at 26.9753 A 1.48851
AFFX-DapX-3_at 42.6275 A 2.67438
AFFX-LysX-5_at 33.387 A 2.15408
AFFX-LysX-M_at 123.338 A 4.54653
AFFX-LysX-3_at 107.05 A 2.75238
AFFX-PheX-5_at 43.6931 A 2.48219
AFFX-PheX-M_at 34.6399 A 2.28331
AFFX-PheX-3_at 463.653 A 9.42123
AFFX-ThrX-5_at 85.5092 A 3.0277
AFFX-ThrX-M_at 32.1851 A 1.81314

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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