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Sample GSM45788 Query DataSets for GSM45788
Status Public on May 14, 2005
Title Mut_M_1
Sample type RNA
Source name GATA-1deltaN mutant E12.5 fetal liver derived megakaryocytes
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaN mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 825.83 P 12.3273
AFFX-BioB-M_at 1169.04 P 14.7109
AFFX-BioB-3_at 1015.02 P 9.58322
AFFX-BioC-5_at 1527.94 P 17.9211
AFFX-BioC-3_at 1718.64 P 17.6273
AFFX-BioDn-5_at 2251.91 P 21.3841
AFFX-BioDn-3_at 5673.38 P 31.5756
AFFX-CreX-5_at 11464.8 P 83.7638
AFFX-CreX-3_at 14305 P 102.004
AFFX-DapX-5_at 29.2647 A 1.99043
AFFX-DapX-M_at 24.3073 A 1.5644
AFFX-DapX-3_at 42.4757 A 3.33921
AFFX-LysX-5_at 32.8403 A 2.54398
AFFX-LysX-M_at 125.51 A 3.49793
AFFX-LysX-3_at 137.929 A 1.79103
AFFX-PheX-5_at 51.7057 A 1.23366
AFFX-PheX-M_at 40.0858 A 2.24286
AFFX-PheX-3_at 492.451 A 15.2011
AFFX-ThrX-5_at 94.8172 A 2.25779
AFFX-ThrX-M_at 38.8255 A 2.14737

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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