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Sample GSM45789 Query DataSets for GSM45789
Status Public on May 14, 2005
Title Mut_M_2
Sample type RNA
Source name GATA-1deltaN mutant E12.5 fetal liver derived megakaryocytes
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaN mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 904.059 P 12.56
AFFX-BioB-M_at 1234.41 P 13.3244
AFFX-BioB-3_at 1066.25 P 9.00373
AFFX-BioC-5_at 1561.79 P 32.3669
AFFX-BioC-3_at 1763.34 P 17.9872
AFFX-BioDn-5_at 2283.63 P 23.4521
AFFX-BioDn-3_at 5970.57 P 72.5425
AFFX-CreX-5_at 11614.2 P 142.674
AFFX-CreX-3_at 14201.8 P 158.722
AFFX-DapX-5_at 37.4442 A 3.45216
AFFX-DapX-M_at 25.8687 A 2.28862
AFFX-DapX-3_at 48.942 A 3.88235
AFFX-LysX-5_at 31.861 A 2.00377
AFFX-LysX-M_at 121.68 A 3.91069
AFFX-LysX-3_at 134.921 A 3.69276
AFFX-PheX-5_at 41.9182 A 2.59165
AFFX-PheX-M_at 35.7056 A 3.35533
AFFX-PheX-3_at 511.953 A 16.4038
AFFX-ThrX-5_at 93.2941 A 5.36175
AFFX-ThrX-M_at 38.7943 A 4.97245

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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