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Sample GSM45790 Query DataSets for GSM45790
Status Public on May 14, 2005
Title WT_P_1
Sample type RNA
Source name wild type E12.5 fetal liver derived megakaryocyte progenitors
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from wild type embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 793.745 P 9.32415
AFFX-BioB-M_at 1101.28 P 11.8521
AFFX-BioB-3_at 1037.16 P 10.425
AFFX-BioC-5_at 1456.84 P 20.5786
AFFX-BioC-3_at 1777.06 P 22.3953
AFFX-BioDn-5_at 2285.17 P 18.7824
AFFX-BioDn-3_at 6261.52 P 46.0506
AFFX-CreX-5_at 11925.9 P 72.6122
AFFX-CreX-3_at 14722.2 P 87.1933
AFFX-DapX-5_at 37.9932 A 2.21556
AFFX-DapX-M_at 22.0776 A 2.18352
AFFX-DapX-3_at 34.4387 A 2.84316
AFFX-LysX-5_at 40.3312 A 2.3736
AFFX-LysX-M_at 142.709 A 1.91675
AFFX-LysX-3_at 74.1693 A 1.72329
AFFX-PheX-5_at 35.0656 A 1.46457
AFFX-PheX-M_at 33.4633 A 2.17065
AFFX-PheX-3_at 245.781 A 12.3456
AFFX-ThrX-5_at 103.355 A 3.56818
AFFX-ThrX-M_at 29.0694 A 2.02865

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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