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Sample GSM45791 Query DataSets for GSM45791
Status Public on May 14, 2005
Title WT_P_2
Sample type RNA
Source name wild type E12.5 fetal liver derived megakaryocyte progenitors
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from wild type embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 712.817 P 8.86324
AFFX-BioB-M_at 1033.93 P 18.5811
AFFX-BioB-3_at 917.676 P 9.50789
AFFX-BioC-5_at 1383 P 12.3167
AFFX-BioC-3_at 1603.57 P 15.4801
AFFX-BioDn-5_at 2144.89 P 25.003
AFFX-BioDn-3_at 5684.95 P 43.4258
AFFX-CreX-5_at 11460 P 69.1217
AFFX-CreX-3_at 14382.8 P 99.4664
AFFX-DapX-5_at 31.9102 A 2.27331
AFFX-DapX-M_at 24.94 A 1.9459
AFFX-DapX-3_at 32.377 A 2.14454
AFFX-LysX-5_at 36.36 A 2.43654
AFFX-LysX-M_at 127.161 A 3.38083
AFFX-LysX-3_at 79.6673 A 1.8551
AFFX-PheX-5_at 43.6356 A 2.62817
AFFX-PheX-M_at 31.9732 A 1.79855
AFFX-PheX-3_at 315.019 A 4.14776
AFFX-ThrX-5_at 99.2085 A 2.64643
AFFX-ThrX-M_at 25.6938 A 2.22081

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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