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Sample GSM45792 Query DataSets for GSM45792
Status Public on May 14, 2005
Title Mut_P_1
Sample type RNA
Source name GATA-1deltaN mutant E12.5 fetal liver derived megakaryocyte progenitors
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaN mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 736.032 P 7.44995
AFFX-BioB-M_at 963.052 P 11.896
AFFX-BioB-3_at 869.428 P 14.9719
AFFX-BioC-5_at 1365.53 P 14.5873
AFFX-BioC-3_at 1685.23 P 14.4285
AFFX-BioDn-5_at 2282.97 P 28.775
AFFX-BioDn-3_at 5871.99 P 48.319
AFFX-CreX-5_at 11418.2 P 65.3476
AFFX-CreX-3_at 13989.9 P 85.5487
AFFX-DapX-5_at 29.1022 A 1.54857
AFFX-DapX-M_at 25.5144 A 1.93482
AFFX-DapX-3_at 40.9715 A 2.23176
AFFX-LysX-5_at 30.6825 A 1.91853
AFFX-LysX-M_at 135.544 A 2.07589
AFFX-LysX-3_at 69.7293 A 3.43407
AFFX-PheX-5_at 48.2561 A 2.58435
AFFX-PheX-M_at 27.6599 A 2.03464
AFFX-PheX-3_at 200.461 A 14.7064
AFFX-ThrX-5_at 93.6976 A 3.37451
AFFX-ThrX-M_at 31.4968 A 2.25763

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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