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Sample GSM45793 Query DataSets for GSM45793
Status Public on May 14, 2005
Title Mut_P_2
Sample type RNA
Source name GATA-1deltaN mutant E12.5 fetal liver derived megakaryocyte progenitors
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaN mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 716.584 P 9.02166
AFFX-BioB-M_at 931.847 P 18.7467
AFFX-BioB-3_at 829.593 P 10.0819
AFFX-BioC-5_at 1340.01 P 21.8185
AFFX-BioC-3_at 1619.01 P 16.8607
AFFX-BioDn-5_at 2264.87 P 36.3446
AFFX-BioDn-3_at 5832.36 P 62.5968
AFFX-CreX-5_at 11670.8 P 99.607
AFFX-CreX-3_at 14410.4 P 99.6275
AFFX-DapX-5_at 36.5621 A 2.83675
AFFX-DapX-M_at 19.5309 A 0.939805
AFFX-DapX-3_at 34.8027 A 2.35681
AFFX-LysX-5_at 28.9228 A 1.98904
AFFX-LysX-M_at 132.142 A 3.12377
AFFX-LysX-3_at 75.598 A 1.80414
AFFX-PheX-5_at 50.2265 A 3.38341
AFFX-PheX-M_at 38.0055 A 2.98007
AFFX-PheX-3_at 261.219 A 9.77167
AFFX-ThrX-5_at 114.608 A 2.42647
AFFX-ThrX-M_at 30.3252 A 1.94536

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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