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Sample GSM45794 Query DataSets for GSM45794
Status Public on May 14, 2005
Title Low_M_1
Sample type RNA
Source name GATA-1deltaNeodeltaHS mutant E12.5 fetal liver derived megakaryocytes
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaNeodeltaHS mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 783.844 P 17.646
AFFX-BioB-M_at 978.845 P 14.9626
AFFX-BioB-3_at 889.091 P 14.7898
AFFX-BioC-5_at 1209.4 P 37.7787
AFFX-BioC-3_at 1621.1 P 34.3232
AFFX-BioDn-5_at 1731.21 P 18.129
AFFX-BioDn-3_at 5578.48 P 69.0153
AFFX-CreX-5_at 10900.7 P 147.989
AFFX-CreX-3_at 14369.6 P 198.539
AFFX-DapX-5_at 43.443 A 2.44852
AFFX-DapX-M_at 26.4683 A 2.24207
AFFX-DapX-3_at 46.399 A 4.349
AFFX-LysX-5_at 59.7911 A 4.58958
AFFX-LysX-M_at 202.879 A 4.85079
AFFX-LysX-3_at 78.3969 A 3.82138
AFFX-PheX-5_at 52.5228 A 2.10402
AFFX-PheX-M_at 39.3189 A 2.44978
AFFX-PheX-3_at 112.854 A 36.7567
AFFX-ThrX-5_at 98.8257 A 4.04275
AFFX-ThrX-M_at 35.9344 A 2.90478

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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