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Sample GSM45795 Query DataSets for GSM45795
Status Public on May 14, 2005
Title Low_M_2
Sample type RNA
Source name GATA-1deltaNeodeltaHS mutant E12.5 fetal liver derived megakaryocytes
Organism Mus musculus
Extracted molecule total RNA
Description E12.5 fetal liver (FL) cells from GATA-1deltaNeodeltaHS mutant embryos were cultured ex vivo in the presence of Tpo for three days. FL-derived megakaryocytes and their progenitors in the culture were separated by BSA density gradient. Megakaryocyte progenitors (“P” population) were further purified by fluorescence-activated cell sorting (FACS) for cells expressing low levels of CD41. Megakaryocytes (“M” population) were further enriched by CD41 antibody coupled with magnetic beads. Total RNA was isolated from either enriched megakaryocyte or progenitor fractions. ~5-15microgram RNA from each sample was submitted to the Dana Farber Cancer Institute Microarray Core Facility for cDNA and cRNA synthesis, labeling, and hybridization with Affymetrix Mouse Genome 430 2.0 chips according to manufacturer’s instructions. All arrays were normalized and analyzed using the dChip program.
Keywords = GATA-1
Keywords = megakaryocyte
Keywords = mouse model
Submission date Mar 23, 2005
Last update date Oct 28, 2005
Contact name Zhe Li
Phone 617-919-2052
Organization name Children's Hospital Boston
Department Division of Hematology/Oncology
Lab Stuart Orkin's Lab
Street address
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL1261
Series (1)
GSE2433 Expression profiling of GATA-1 wild type and mutant fetal megakaryocytes

Data table header descriptions
VALUE Normalized dChip1.3 expression value
ABS_CALL Presence/absence call
SE Standard Error

Data table
AFFX-BioB-5_at 915.758 P 13.3322
AFFX-BioB-M_at 1215.98 P 12.675
AFFX-BioB-3_at 1075.15 P 15.1532
AFFX-BioC-5_at 1563.66 P 33.4851
AFFX-BioC-3_at 1959.53 P 29.8276
AFFX-BioDn-5_at 2372.26 P 29.7441
AFFX-BioDn-3_at 6310.48 P 86.8746
AFFX-CreX-5_at 11819.9 P 87.084
AFFX-CreX-3_at 14589 P 111.031
AFFX-DapX-5_at 36.8152 A 2.34114
AFFX-DapX-M_at 21.9947 A 2.3771
AFFX-DapX-3_at 41.3602 A 1.8172
AFFX-LysX-5_at 49.5986 A 2.40265
AFFX-LysX-M_at 169.437 A 2.57888
AFFX-LysX-3_at 73.6688 A 4.95668
AFFX-PheX-5_at 52.7284 A 2.87198
AFFX-PheX-M_at 30.3483 A 3.81801
AFFX-PheX-3_at 151.6 A 35.8946
AFFX-ThrX-5_at 87.2344 A 2.84201
AFFX-ThrX-M_at 40.0635 A 2.09872

Total number of rows: 45101

Table truncated, full table size 1285 Kbytes.

Supplementary data files not provided

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