|
| Status |
Public on Sep 27, 2010 |
| Title |
Castration-recurrent; Biological replicate #2 |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
326133 |
| Tag Length |
17 |
| |
|
| Source name |
In vivo LNCaP Hollow Fibre model
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Stage in hormonal progression is castration-recurrent; Biological replicate #2
cell line: LNCaP
disease state: prostate cancer, castration-recurrent
|
| Biomaterial provider |
American Type Culture Collection
|
| Treatment protocol |
Fibres containing LNCaP cells were removed from mouse 15N at the castration-recurrent stage of hormonal progression of prostate cancer. Samples were retrieved 72 days following surgical castration of the mouse.
|
| Growth protocol |
LNCaP cells were grown in hollow fibres in a mouse.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated immediately from cells harvested from the in vivo Hollow Fibre model using TRIZOL Reagent following the manufacturer’s instructions. Genomic DNA was removed from RNA samples with DNaseI.
|
| |
|
| Description |
LongSAGE libraries were constructed with 5ug of total RNA using the Invitrogen I-SAGE Long kit.
|
| |
|
| Submission date |
Oct 02, 2009 |
| Last update date |
Sep 26, 2010 |
| Contact name |
Marianne D. Sadar |
| Organization name |
British Columbia Cancer Agency
|
| Department |
Genome Sciences Centre
|
| Street address |
675 W. 10th Ave
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1485 |
| Series (1) |
| GSE18402 |
LNCaP Atlas: Gene expression associated with in vivo progression to castration-recurrent prostate cancer |
|
