|
| Status |
Public on Jun 06, 2021 |
| Title |
KOTFH-1: Rictor-null Tfh, biological rep.1 |
| Sample type |
RNA |
| |
|
| Source name |
Rictor-null, LCMV Armstrong D60
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lymphocyte
developmental stage: memory stage of CD4 T cells in LCMV acute infection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Cat. AM1792, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 with constant rotation at the speed of 60 rpm.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Tfh cells in spleen of Rictor-null mice at day 60-post LCMV Armstrong infection
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Jun 04, 2020 |
| Last update date |
Jun 06, 2021 |
| Contact name |
Yifei Wang |
| E-mail(s) |
yfwangsophia@163.com
|
| Phone |
86-15088062080
|
| Organization name |
Third Military Medical University
|
| Department |
Institute of Immunology
|
| Street address |
Gaotanya Street
|
| City |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE151800 |
Gene expression data from memory Rictor-/- and WT Tfh and Th1 cells in LCMV acute infection |
|
