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Status |
Public on Jun 06, 2021 |
Title |
WTTFH-1: Wild-type Tfh, biological rep.1 |
Sample type |
RNA |
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Source name |
Wild-type, LCMV Armstrong D60
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Organism |
Mus musculus |
Characteristics |
tissue: lymphocyte developmental stage: memory stage of CD4 T cells in LCMV acute infection
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Cat. AM1792, Ambion).
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 with constant rotation at the speed of 60 rpm.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression data from Tfh cells in spleen of wild-type mice at day 60-post LCMV Armstrong infection
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Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and RMA as normalization method.
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Submission date |
Jun 04, 2020 |
Last update date |
Jun 06, 2021 |
Contact name |
Yifei Wang |
E-mail(s) |
yfwangsophia@163.com
|
Phone |
86-15088062080
|
Organization name |
Third Military Medical University
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Department |
Institute of Immunology
|
Street address |
Gaotanya Street
|
City |
Chongqing |
ZIP/Postal code |
400038 |
Country |
China |
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Platform ID |
GPL1261 |
Series (1) |
GSE151800 |
Gene expression data from memory Rictor-/- and WT Tfh and Th1 cells in LCMV acute infection |
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