|
| Status |
Public on Oct 30, 2009 |
| Title |
Frontonasal mass prominence, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Stage 18 chicken embryos
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Mesenchyme plus epithelium
genotype: Unknown
age: Hamburger Hamilton stage 18
|
| Treatment protocol |
No treatment was done to the embryos. These are normal tissues.
|
| Growth protocol |
Embryos were incubated at 38 degrees C until they reached Hamburger Hamilton Stage 18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit was used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to two-cycle Affymetrix protocol from 500 ng total RNA (GeneChip Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed as described in the GeneChip Expression Analysis Technical Manual by the London Regional Genomics Centre, London, Ontario, Canada
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000
|
| Description |
Gene expression data from stage 18 frontonasal mass prominences, between the nasal pits
|
| Data processing |
The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and Scaling within 3 fold of each other as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Oct 08, 2009 |
| Last update date |
Oct 08, 2009 |
| Contact name |
Joy Richman |
| E-mail(s) |
richman@dentistry.ubc.ca
|
| Organization name |
University of British Columbia
|
| Department |
Oral Health Sciences
|
| Street address |
2350 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE18477 |
Normal expression of facial prominences in stage 18 chicken embros |
|
