|
Status |
Public on Jul 13, 2010 |
Title |
FOXA2_NORM_rep1 |
Sample type |
RNA |
|
|
Source name |
FOXA2_NORM
|
Organism |
Mus musculus |
Characteristics |
tissue: prostate tumor strain: C57BL6 sex: male cell type: TRAMP-C
|
Treatment protocol |
TRAMP-C cells were transfected with pcDNA, or FoxA2, or HIF-1a, or HIF-1a + FoxA2 using Amax Nucleofector in duplicate. 48 hour post transfection, cells were treated with normoxia or 1% hypoxia for 12 h before isolation of total RNA. 500 ng of total RNA was used for synthesis of biotin-labeled cRNA using an RNA amplification kit (Ambion). The biotinylated cRNA is labeled by incubation with streptavidin-Cy3 to generate probe for hybridization with the Mouse-6 Expression BeadChip (Illumina) that represents 48K mouse gene transcripts.
|
Growth protocol |
TRAMP-C cells were isotated from the prostate tumor of TRAMP mice, which is C57BL6. The TRAMP-C cells are wild-type.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
streptavidin Cy3
|
Label protocol |
As suggested by illumina
|
|
|
Hybridization protocol |
As recommended by illumina
|
Scan protocol |
As suggested by illumina using the illumina scanner
|
Description |
4323501007_C
|
Data processing |
Raw data filtered on P value for <0.05 with the remaining data normalised using the affy tools R bioconductor normalize.quantiles routine
|
|
|
Submission date |
Oct 08, 2009 |
Last update date |
Jul 02, 2010 |
Contact name |
Roy M Williams |
E-mail(s) |
rwillia@scripps.edu
|
Organization name |
The Scripps Research Institute
|
Department |
Regenerative Medicine
|
Lab |
Loring
|
Street address |
3030 Science Park Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE18478 |
Siah2-dependent concerted activity of HIF and FoxA2 regulates formation of neuroendocrine prostate tumors |
|