|
| Status |
Public on Jun 01, 2021 |
| Title |
WT Br female rep1 |
| Sample type |
RNA |
| |
|
| Source name |
wild type female rest of brain
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
|
| Treatment protocol |
mice were sacrificed and their brains removed immediately afterwards; while fresh, extracted brains were manually cleaned of brain stem tissue remnants by gross dissection, and split into cerebellum and rest-of-brain. Each sample was then placed inside an individual 15-ml conical, put dry-ice to snap-freeze, and archived in -80degC. To prevent cross-contamination and tissue degradation due to delayed processing, mice were dissected one at a time with a single-use scalpel blade each.
|
| Growth protocol |
Animals were maintained under regular housing conditions prior to sampling
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol from 50-100 mg of tissue
|
| Label |
biotin
|
| Label protocol |
One hundred ng of total RNA was amplified as directed in the Affymetrix 3' IVT Plus kit protocol.
|
| |
|
| Hybridization protocol |
12.5 μg of amplified biotin-aRNAs were fragmented and 10 μg were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification per the GeneChip Hybridization, Wash and Stain Kit and user manual.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
gene expression data from female rest of brain (whole brain minus the cerebellum)
|
| Data processing |
Data was obtained using the TAC 4.0 Software using the MAS5 algorithm to generate .CHP files.
|
| |
|
| Submission date |
Jun 10, 2020 |
| Last update date |
Jun 02, 2021 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE152224 |
Mutations on a novel brain-specific isoform of PGC1α leads to extensive upregulation of neurotransmitter-related genes and sexually dimorphic motor deficits in mice |
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