|
| Status |
Public on Oct 10, 2009 |
| Title |
mES E2-10nM |
| Sample type |
RNA |
| |
|
| Source name |
E2-10nM
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6
cell type: embryonic stem cell
|
| Treatment protocol |
Chemicals were treated into the culture media at the iniciated stage after removing LIF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Expose 10nM estradiol for 48hours
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Oct 09, 2009 |
| Last update date |
Oct 09, 2009 |
| Contact name |
Sone Hideko |
| E-mail(s) |
hsone@nies.go.jp
|
| Phone |
81298502420
|
| Organization name |
National Institute for Environmental Studies
|
| Department |
Center for Environmental Risk Research
|
| Lab |
Environmental Exposure Research Section
|
| Street address |
onogawa16-2
|
| City |
tsukuba |
| State/province |
ibaraki |
| ZIP/Postal code |
305-8506 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE18503 |
Effects of environmental chemicals on development of neuronal cell lineages derived from mouse ES cells |
|
