|
Status |
Public on Oct 10, 2009 |
Title |
mES Cyclopamine-0.01mM |
Sample type |
RNA |
|
|
Source name |
Cyclopamine-0.01mM
|
Organism |
Mus musculus |
Characteristics |
genetic background: C57BL/6 cell type: embryonic stem cell
|
Treatment protocol |
Chemicals were treated into the culture media at the iniciated stage after removing LIF.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Expose 0.01mM cyclopamine for 48hours
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Oct 09, 2009 |
Last update date |
Oct 09, 2009 |
Contact name |
Sone Hideko |
E-mail(s) |
hsone@nies.go.jp
|
Phone |
81298502420
|
Organization name |
National Institute for Environmental Studies
|
Department |
Center for Environmental Risk Research
|
Lab |
Environmental Exposure Research Section
|
Street address |
onogawa16-2
|
City |
tsukuba |
State/province |
ibaraki |
ZIP/Postal code |
305-8506 |
Country |
Japan |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE18503 |
Effects of environmental chemicals on development of neuronal cell lineages derived from mouse ES cells |
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