|
| Status |
Public on Oct 01, 2010 |
| Title |
E14.5 radial glia cells rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic forebrain, radial glia cells, FACS isolated, CD133+/CD24neg/CD45neg/CD34neg
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
developmental stage: embryonic day 14.5
cell source: FACS sorted from tissue
facs phenotype: D133+/CD24neg/CD45neg/CD34neg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and purified with QIAgen's RNeasy Micro Kit including DNase I treatment
|
| Label |
biotin
|
| Label protocol |
RNA amplification and biotin-labeling were achieved with the TargetAmp 2-Round Biotin-aRNA Amplification Kit (Epicentre)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
E14_02
|
| Data processing |
Raw data was background-corrected and summarized using BeadStudio software (Illumina) and further processed with the Bioconductor/Lumi package; data were normalized applying quantile normalization and variance stabilization (function vst).
|
| |
|
| Submission date |
Oct 13, 2009 |
| Last update date |
Oct 13, 2009 |
| Contact name |
Ulrike A. Nuber |
| Organization name |
Lund University
|
| Street address |
Klinikgatan 26
|
| City |
Lund |
| ZIP/Postal code |
SE-22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE18528 |
Comparative analysis of ependymal cells from the lateral ventricular wall and the spinal cord of adult mice |
|
