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Sample GSM465081 Query DataSets for GSM465081
Status Public on Oct 30, 2009
Title Sham 1
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Sham treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 277.0916
ILMN_2721178 36.27205
ILMN_3033922 2062.002
ILMN_3092673 7767.391
ILMN_2816356 95.9156
ILMN_2808939 909.9357
ILMN_2634564 493.0375
ILMN_2737647 4.442258
ILMN_2734484 456.8343
ILMN_2952292 153.2281
ILMN_2699078 0.5228447
ILMN_1213681 525.2144
ILMN_2735413 0.6494336
ILMN_2735415 7.449284
ILMN_2891688 706.0333
ILMN_2637698 2611.85
ILMN_2674228 661.6713
ILMN_2601546 1.615994
ILMN_1230831 -3.224396
ILMN_2848071 17.97542

Total number of rows: 25697

Table truncated, full table size 555 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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