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Sample GSM465082 Query DataSets for GSM465082
Status Public on Oct 30, 2009
Title ODN2395 1
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description ODN2395 treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 232.6272
ILMN_2721178 28.97703
ILMN_3033922 1402.555
ILMN_3092673 4839.585
ILMN_2816356 132.4194
ILMN_2808939 780.9254
ILMN_2634564 349.0361
ILMN_2737647 1.911431
ILMN_2734484 312.8216
ILMN_2952292 125.008
ILMN_2699078 -1.564827
ILMN_1213681 531.8379
ILMN_2735413 5.567564
ILMN_2735415 22.42086
ILMN_2891688 389.9468
ILMN_2637698 2714.401
ILMN_2674228 339.1496
ILMN_2601546 7.742116
ILMN_1230831 9.524928
ILMN_2848071 17.97286

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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