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Sample GSM465083 Query DataSets for GSM465083
Status Public on Oct 30, 2009
Title Pam2 1
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Pam2CSK4 treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 196.6697
ILMN_2721178 27.09277
ILMN_3033922 1960.915
ILMN_3092673 7459.818
ILMN_2816356 90.67646
ILMN_2808939 1031.846
ILMN_2634564 580.3749
ILMN_2737647 12.08511
ILMN_2734484 552.8417
ILMN_2952292 181.0058
ILMN_2699078 -15.73669
ILMN_1213681 544.7144
ILMN_2735413 3.291429
ILMN_2735415 4.160456
ILMN_2891688 941.2988
ILMN_2637698 2082.679
ILMN_2674228 751.2699
ILMN_2601546 -0.1789941
ILMN_1230831 0.8197365
ILMN_2848071 9.657493

Total number of rows: 25697

Table truncated, full table size 557 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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