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Sample GSM465084 Query DataSets for GSM465084
Status Public on Oct 30, 2009
Title ODN/Pam 1
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Combination treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 208.118
ILMN_2721178 97.87438
ILMN_3033922 1225.235
ILMN_3092673 4226.959
ILMN_2816356 83.31677
ILMN_2808939 441.9597
ILMN_2634564 506.5463
ILMN_2737647 55.31453
ILMN_2734484 199.7518
ILMN_2952292 84.92068
ILMN_2699078 3.774641
ILMN_1213681 481.9898
ILMN_2735413 -14.04023
ILMN_2735415 -9.923738
ILMN_2891688 502.2536
ILMN_2637698 2488.749
ILMN_2674228 422.9207
ILMN_2601546 24.41865
ILMN_1230831 21.88207
ILMN_2848071 -4.901948

Total number of rows: 25697

Table truncated, full table size 555 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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