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Sample GSM465087 Query DataSets for GSM465087
Status Public on Oct 30, 2009
Title Pam2 2
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Pam2CSK4 treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 190.2441
ILMN_2721178 39.14773
ILMN_3033922 1596.034
ILMN_3092673 6772.942
ILMN_2816356 88.06786
ILMN_2808939 947.1526
ILMN_2634564 654.4203
ILMN_2737647 24.66633
ILMN_2734484 446.5999
ILMN_2952292 183.1996
ILMN_2699078 -2.793392
ILMN_1213681 500.9201
ILMN_2735413 0.03385268
ILMN_2735415 18.4783
ILMN_2891688 951.7684
ILMN_2637698 2170.949
ILMN_2674228 871.2108
ILMN_2601546 20.22015
ILMN_1230831 17.47933
ILMN_2848071 18.17465

Total number of rows: 25697

Table truncated, full table size 555 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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