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Sample GSM465088 Query DataSets for GSM465088
Status Public on Oct 30, 2009
Title ODN/Pam 2
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Combination treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 226.9685
ILMN_2721178 17.93917
ILMN_3033922 1905.716
ILMN_3092673 7095.37
ILMN_2816356 115.0957
ILMN_2808939 1003.377
ILMN_2634564 520.6379
ILMN_2737647 10.16019
ILMN_2734484 530.3234
ILMN_2952292 163.6431
ILMN_2699078 -0.5979289
ILMN_1213681 459.1455
ILMN_2735413 8.931188
ILMN_2735415 25.8573
ILMN_2891688 711.7257
ILMN_2637698 2424.818
ILMN_2674228 593.6665
ILMN_2601546 9.119667
ILMN_1230831 5.577089
ILMN_2848071 21.49825

Total number of rows: 25697

Table truncated, full table size 556 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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