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Sample GSM465089 Query DataSets for GSM465089
Status Public on Oct 30, 2009
Title Pam2 3
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description Pam2CSK4 treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 252.7641
ILMN_2721178 33.36438
ILMN_3033922 1862.216
ILMN_3092673 7116.725
ILMN_2816356 131.3788
ILMN_2808939 1136.389
ILMN_2634564 611.3875
ILMN_2737647 13.71749
ILMN_2734484 377.0559
ILMN_2952292 189.0496
ILMN_2699078 -2.055378
ILMN_1213681 724.3013
ILMN_2735413 18.51847
ILMN_2735415 1.911642
ILMN_2891688 751.3922
ILMN_2637698 2462.757
ILMN_2674228 867.8027
ILMN_2601546 22.51356
ILMN_1230831 12.34888
ILMN_2848071 12.90412

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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