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Sample GSM465577 Query DataSets for GSM465577
Status Public on Oct 28, 2009
Title Nucleus accumbens repeated wd + acute cocaine 1
Sample type RNA
 
Source name Mouse nucleus accumbens 1 hr
Organism Mus musculus
Characteristics strain: C57BL/6J
agent: 7 treatments 20 mg/kg cocaine-HCl + 7 treatments saline + 1 challenge treatment 20 mg/kg cocaine-HCl, i.p.
age: 8-10 week old
tissue: Bilateral, 1mm-thick, 15-gauge punches, pooled together from 4 animals
Treatment protocol Animals were treated daily with either 'saline' (15 treatments saline, i.p.), 'acute' cocaine (14 treatments saline + 1 treatment 20 mg/kg cocaine-HCl, i.p.), 'repeated + acute' cocaine (7 treatments saline + 8 treatments 20 mg/kg cocaine-HCl, i.p.) or 'repeated withdrawal + acute' cocaine (7 treatments 20 mg/kg cocaine-HCl + 7 treatments saline + 1 challenge treatment 20 mg/kg cocaine-HCl, i.p.) and were sacrificed 1 hour after the final treatment.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
Label Biotin
Label protocol Standard Illumina labelling protocol
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing The data were normalized using quantile normalization with Beadstudio software from illumina
 
Submission date Oct 27, 2009
Last update date Oct 27, 2009
Contact name Ian Sutherland Maze
E-mail(s) ian.maze@mssm.edu
Phone 212-659-5934
Organization name Mount Sinai School of Medicine
Department Neuroscience
Lab Nestler Lab
Street address 1425 Madison Ave.
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6887
Series (1)
GSE18751 Genomic sensitization in response to G9a repression following repeated cocaine exposure

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 50.76097 0
ILMN_1251402 -2.425224 0.5764192
ILMN_3122480 -6.06739 0.7882096
ILMN_1230863 -6.80283 0.8176856
ILMN_2599935 287.3718 0
ILMN_1249762 14.76685 0.04585153
ILMN_2653194 8954.437 0
ILMN_1227991 -9.437122 0.9213974
ILMN_2445236 104.412 0
ILMN_2675543 -3.956727 0.6681223
ILMN_2635314 -10.94242 0.9530568
ILMN_1212991 258.2589 0
ILMN_2686883 23.77217 0
ILMN_1223534 -1.398194 0.5207424
ILMN_2514305 64.12063 0
ILMN_2751818 1.876915 0.3646288
ILMN_1217583 6.257704 0.191048
ILMN_1222597 72.13498 0
ILMN_1242561 12.25888 0.07314411
ILMN_2728634 26.544 0

Total number of rows: 45281

Table truncated, full table size 1262 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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